Insert the information in the form and the calculator will determine the isoelectric point.

What is the isoelectric point?

The isoelectirc point is the PH at which a particular molecule carries no net electrical charge. The net charge on the molecule is affected by the pH of it's surrounding environment and can become more negative or positive due to the gain or loss of protons.

How to calculate isoelectric point?

To calculate isoelectric point you should use the following formula:
Isoelectirc point = (Pka1 + Pka2) / 2

Why use isoelectric point?

The isoelectric point is important in protein purification because it represents the pH where solubility is typically minimal.